IJPNI. 2016;3(1):3-3 doi: 10.15171/ijpni.2016.03
antimicrobial action of Bixa orellana L.
orellana L. is a plant that grows in Cuba, which is held to have
Material and methods: An
experimental study was carried out to evaluate the in vitro antimicrobial
action of 50% fluid extract and 10% and 20% tinctures made of the leaves of the
species Bixa orellana L. The method of macrodilution in broth was used and minimal
concentration with antimicrobial activity, expressed in dilutions, was
determined. Strains of international reference were used.
Results: The tinctures at 10%
and 20% of the leaf showed bactericidal action against Staphylococcus aureus at a concentration of 1:8 and 1:32
respectively, whereas only 10% tincture showed antimicrobial activity against Escherichia
coli at a concentration of 1:8, however, they were not active against Pseudomona
aeruginosa and Candida albicans. As to 50 % fluid extract, it only
showed bactericidal activity against Staphylococcus aureus at a concentration of 1:4.
Conclusion: Both, fluid extract at 50 % and tinctures at 10% and 20% of the leaf
were active against Staphylococcus aureus at lower concentrations compared to the rest of microorganisms, but they had no antimicrobial
activity against Candida albicans or Pseudomona aeruginosa. 50% fluid
extract showed antimicrobial activity only against Staphylococcus aureus. Tincture
at 10% confirmed activity against Staphylococcus
and Escherichia coli. Tincture at 20%was of use only against Staphylococcus aureus. Staphylococcus
aureus turned out to be the most sensitive germ to the pharmaceutical
forms studied at lower concentrations. Tincture at 10% showed a wider spectrum
of antimicrobial activity.