International Journal of Phytocosmetics and Natural Ingredients

An International Journal of Integrated Sciences
   ISSN: 2374-0639
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Submitted: 21 Apr 2015

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IJPNI. 2014, Volume 1; 1.

Original Research
Antioxidant activity of phenolic compounds from whole cottonseed by-product

Diana Yamile Gallego Villa, Anna Lisa Piccinelli, Luigi Russo, Luca Rastrelli *

1 Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Cra 30 Nº 45-03, Bogotá, Colombia
2 Dipartimento di Farmacia, University of Salerno, Via Ponte don Melillo, 84084, Fisciano (SA), Italy

Cottonseeds are fed to high-producing dairy cows as a source of fat and highly-digestible fiber. Previously we reported the isolation and structure characterization of different phenolic compounds, quercetin, kaemferol and benzoic acid derivatives (1-9) from whole cottonseed by-product. In this paper the antioxidant activity of all isolated compounds was determined by measuring free radical scavenging effects using the Trolox equivalent antioxidant capacity assay and the coupled oxidation of β-carotene and linoleic acid (autoxidation assay). The range of phenolic compounds present in WCS (1.90 g/kg), assayed by the Folin-Ciocalteu method, and their antioxidant properties provides a valuable database for possible nutritional studies on the impact of diet on animal health and productivity.

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