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International Journal of Phytocosmetics and Natural Ingredients

An International Journal of Integrated Sciences
   ISSN: 2374-0639
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Submitted: 21 Apr 2015

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IJPNI. 2014, Volume 1; 4.

Original Research
Chemical composition, anti-dermatophytic activity, antioxidant and total phenolic content within the leaves essential oil of Vitex trifolia

W. Radhapiyari Devi * , Chingakham B. Singh


Abstract
This study was carried out to determine the contents of total phenol as well as antioxidant activity, the chemical composition and anti-dermatophytic activity of the Vitex trifolia (Family: Lamiaceae) essential oil of the leaves. Hydro-distillation of the V. trifolia was carried out using Clavenger Vendor to obtain the volatile oil. Total phenolic content and antioxidant activity were determined using the Folin-Ciocalteau reagent and DPPH radical scavenging assay test by spectrophotometric methods. Gas chromatography- mass spectrometric (GC-MS) analyses (BP - 1 chrompack capillary column) of the essential oil was performed and its composition determined. The in-vitro anti-dermatophytic activity was evaluated both by agar diffusion and microbroth dilution methods against Candida albicans ATCC 90029, C. albicans ATCC 1162, C. albicans ATCC Y-9-19, C. parapsilosis ATCC 20019, C. glabrata ATTC 91030, C. krusei ATCC 6258, C. krusei ATCC 71061-1113 and C. kefyr ATCC 1110. The fresh leaves were yielded 0.21% of yellowish colour essential oil. By GC-MS analysis of the essential oil, 50 constituents were identified. 1R-α-pinene, Ocimene, 3-carene, Toluene, p-ethyl-, Eucalyptol and 3-methoxy-5-methylphenol were the major constituents. The V. trifolia contained appreciable levels of amount of total phenolic content (74.66μg GAE/100mg) of the fresh weight and the DPPH free radical scavenging activity (the IC50 = 5.2 μg/ml). The anti-dermatophytic activity showed that essential oil had some antifungal activity on the tested Candida in the concentration 500 μg/ml. The highest antifungal activity was C. krusei ATCC 6258 and C. kefyr ATCC 1110 (zone diameter 27.97±0.4 mm and 23.88±0.8 mm) respectively and C. krusei ATCC 6258 (MIC = 1.953125 μl/ml).




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